Class NEET 2026 ALL Q #2059
KNOWLEDGE BASED
APPLY
4 Marks 2026 NTA-RE-NEET-2026 MCQ SINGLE
Match List-I with List-II.
List-I:
A. Transformation
B. Cloning site
C. Selection
D. Ori
List-II:
I. Restriction enzyme
II. Transfer DNA to host bacteria
III. Replication
IV. Antibiotic
Choose the correct answer from the options given below:
(A) A-IV, B-I, C-III, D-II
(B) A-II, B-I, C-IV, D-III
(C) A-I, B-II, C-IV, D-III
(D) A-III, B-IV, C-II, D-I
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Correct Answer: B

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Detailed Solution

Step 1: Analyze List-I and List-II

We need to match the biotechnological terms in List-I with their respective functions or definitions in List-II.

Step 2: Evaluate each term

A. Transformation: This is the process of introducing foreign DNA into a host cell (bacteria). Matches with II (Transfer DNA to host bacteria).

B. Cloning site: This is the specific recognition sequence where a restriction enzyme cuts the DNA to allow insertion. Matches with I (Restriction enzyme).

C. Selection: This involves using markers, such as antibiotic resistance genes, to identify transformed cells. Matches with IV (Antibiotic).

D. Ori (Origin of Replication): This is the sequence where replication starts. Matches with III (Replication).

Step 3: Final Mapping

The correct sequence is: A-II, B-I, C-IV, D-III.

Final Answer: Option (B)

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Pedagogical Audit
Bloom's Analysis: This is an APPLY question because the student must relate specific biotechnological terminology to their functional roles in recombinant DNA technology.
Knowledge Dimension: CONCEPTUAL
Justification: The question tests the understanding of core concepts in Biotechnology: Principles and Processes, requiring the student to synthesize knowledge of vector components and transformation techniques.
Syllabus Audit: In the context of NEET, this is classified as KNOWLEDGE (Textbook). This question directly assesses the student's grasp of standard definitions and functional components of cloning vectors as outlined in the NCERT Biology curriculum.

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