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Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells.
Reason R: Isolation of genetic material needs disruption of cells.
In the light of the above statements, choose the most appropriate answer from the options given below:
APPLY COMPETENCY 4 Marks
Concept Application
50%
Calculation / Logic
50%
Target Level
MEDIUM
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Q: Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells.
Reason R: Isolation of genetic material needs disruption of cells.
In the light of the above statements, choose the most appropriate answer from the options given below:

Question Analysis & Solution

Detailed Solution

Step 1: Analyze Assertion A

Assertion A states that lysozyme is used to disrupt bacterial cells and cellulase is used for plant cells. In recombinant DNA technology, the first step is the isolation of DNA. Since bacterial cells have a cell wall made of peptidoglycan, lysozyme is the appropriate enzyme to break it down. Similarly, plant cells have a cell wall made of cellulose, requiring cellulase for degradation. Thus, Assertion A is correct.

Step 2: Analyze Reason R

Reason R states that the isolation of genetic material needs the disruption of cells. This is a fundamental principle of biotechnology. To access the DNA contained within the nucleus or cytoplasm, the cell membrane and cell wall must be ruptured. Thus, Reason R is correct.

Step 3: Determine the Relationship

The reason why we use specific enzymes like lysozyme or cellulase is precisely because the isolation of genetic material requires the disruption of the cell wall. Therefore, the use of these specific enzymes is a direct consequence of the need to isolate genetic material. Thus, Reason R is the correct explanation of Assertion A.

Final Answer: Both A and R are correct and R is the correct explanation of A

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