Match List-I with List-II. List-I: A. Transformation B. Cloning site C. Selection D. Ori List-II: I. Restriction enzyme II. Transfer DNA to host bacteria III. Replication IV. Antibiotic Choose the correct answer from the options given below:

Which of the following statements about the reabsorption process in Henle's loop are correct? (a) The descending limb of Henle's loop is permeable to water but almost impermeable to electrolytes. (b) Urine gets concentrated in Henle's loop. (c) Reabsorption of $Na^{+}$ and water takes place in Henle's loop. (d) Active or passive transport of electrolytes occurs in the ascending limb of Henle's loop. Choose the correct answer from the options given below:

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R. Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells. Reason R: Isolation of genetic material needs disruption of cells. In the light of the above statements, choose the most appropriate answer from the options given below:

The correct statement about peptides and proteins is

A protein undergoes reversible thermal denaturation from its initial state N to denatured state D according to $N \rightleftharpoons D$. At $60^{\circ}C$, the concentrations of both N and D are equal at equilibrium, and the standard enthalpy change of denaturation is $666~kJ~mol^{-1}$. The standard entropy change ($\Delta S^{\circ}$ in $kJ~K^{-1}~mol^{-1}$) of the protein upon denaturation at $60^{\circ}C$ is closest to