Class NEET 2026 ALL Q #2054
COMPETENCY BASED
APPLY
4 Marks 2026 NTA-RE-NEET-2026 MCQ SINGLE
The inactive form of Bt toxin is converted to the active form in the insect gut
(A) by nucleases
(B) due to alkaline pH
(C) due to acidic pH
(D) by proteases
Correct Answer: B

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Detailed Solution

Step 1: Understanding Bt Toxin

The Bacillus thuringiensis (Bt) bacterium produces insecticidal crystal proteins known as Cry proteins. These proteins exist in an inactive protoxin form within the bacterium.

Step 2: Mechanism of Activation

When an insect ingests the inactive Bt toxin, it enters the midgut of the insect. The midgut environment of the target insect is characterized by a high alkaline pH.

Step 3: Conversion Process

The alkaline pH of the gut solubilizes the crystals, allowing the inactive protoxin to be converted into its active toxic form. This active toxin then binds to the epithelial cells of the midgut, causing cell lysis and eventually the death of the insect.

Final Answer: due to alkaline pH

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Pedagogical Audit
Bloom's Analysis: This is an APPLY question because it requires the student to recall specific biological mechanisms and apply that knowledge to explain the activation process of a toxin in a specific physiological environment.
Knowledge Dimension: CONCEPTUAL
Justification: The question tests the understanding of the relationship between environmental conditions (pH) and biochemical activation, which is a core concept in Biotechnology.
Syllabus Audit: In the context of NEET, this is classified as COMPETENCY. This question is a standard NCERT-based inquiry frequently appearing in competitive biology exams to test conceptual clarity on Biotechnology applications.